PROTEIN & ANTIBODy Production UNIT

    Group Leader: Alessandra Fucci (PhD)

    Senior advisor: Maria Luisa Nolli (PhD)

    Staff:  Assunta Riccio (PhD)

               Egildo Luca D’Andrea (PhD)

               Simona Giacobbe (PhD)

               Ylenia Abruzzese (BSc)


    Main activity areas

    The PF team has a consolidated experience in the development, production, purification  and characterization of recombinant proteins, antibodies and antibody-derivatives, as well as in the customized  monoclonal antibodies generation through hybridoma technology.

    PF activities also include design and validation of immunoassays (ELISA), affinity and binding analyses by SPR, preparation of tech-transfer protocols for biosimilar industrialization, and recombinant enzymes manufacturing.

    Main Technologies 

    PF has at its disposal multiple host strains and cell lines, as well as a large library of IP-free expression vectors for the production of recombinant proteins and antibodies. 

    PF performance is ensured by the design of project-specific and tailored strategies and by the use of cutting-edge tools and methods. In addition, advanced analytical techniques, such as SPR, HPLC and LC-MS/MS, together with the standard biochemical analyses, are available for proteins and antibodies characterization.

    Main Services

    Recombinant Proteins and Antibodies production and characterization

    • Gene synthesis (with or without codon optimization) and expression plasmid preparation;
    • Expression testing in the selected host (E. coli, P. pastoris, or Mammalian cells)
    • Expression optimization and set-up of the purification method
    • Scale-up of protein expression and purification,
    • Analysis of quantity, purity and integrity (UV-Vis, SDS-PAGE, HPLC, and Western Blot)
    • Characterization (HPLC, LC-MS/MS), binding and kinetics studies (SPR)
    • Endotoxin testing and removal
    • Protein and Antibody Labeling

    Schermata 2023 09 22 alle 15.21.13


    Custom Antibodies development

    • Immunization strategy design
    • Antigen design and production (optional)
    • Immunization (Mice, Rats, Rabbits) and serum antibody titration
    • Hybridoma generation and screening (for Monoclonal Ab production)
    • Stabilization and banking of the monoclonal culture
    • Purification of the antibodies (0.1-100 mg)
    • Ab characterization (isotyping, SDS-PAGE, ELISA, HPLC, SPR)

    Schermata 2023 09 22 alle 15.21.13


    Immunoassay development

    • Antibody selection and pairing
    • Generation of calibration curves for quantitative applications
    • Assay optimization, qualification, and analytical validation
    • Manufacturing and delivery of the assay kit
    • Fit-for-purpose ELISA modification of existing assays

    Schermata 2023 09 22 alle 15.21.13

    Biogem S.c.a r.l. P.Iva 02071230649
    Via Camporeale Area P.I.P. Ariano Irpino (AV) Italy
    Tel. +39 0825 881811 - Fax +39 0825 881812 -

    © 2024 Biogem scarl. All Rights Reserved.