ANIMAL MODELS CORE FACILITY (AMCF)

(Supervisor: Mario De Felice)

ANIMAL MODELS CORE FACILITY (AMCF)

Members

Supervisor:              Prof. Mario De Felice, MD
Molecular Biology:    Dr Pina Marotta, PhD; Dr. Nicola Antonino Russo, PhD.
Cell Biology:             Dr. Marina Marotta; Dr. Carla Reale, PhD; Dr. Filomena Russo
Embryology:             Dr. Luca Roberto
Zebrafish modelling:  Dr. Immacolata Porreca, PhD

Staff Contacts:
 
Dr. Pina Marotta
Mice unit
pina.marotta@biogem.it
 
Dr. Immacolata Porreca
Zebrafish unit
immacolata.porreca@biogem.it

 

I. Transgenic Mice Service

Genetically modified mouse models are one of the most powerful tools in scientific areas such as cancer, drug discovery and development biology. Nowadays it is possible to remove, replace and insert any kind of sequence in the mouse genome. Biogem offers quality custom generation of a wide range of mouse models, via homologous recombination in embryonic stem (ES) cells, using a variety of advanced molecular biology technologies, enabling you to obtain the precise models you need.

Our services include:
  • Targeting strategy design.
  • Targeting vector construction.
  • Electroporation of mouse embryonic stem (ES) cells.
  • Assessing ES cells for homologous recombination.
  • Generating chimeric mice by morula or blastocyst injection.
  • Assessing germline transmission.
  • Breeding chimeric mice to heterozygosity.

Our services can be ordered together or individually depending on the investigator's needs. Moreover, we can generate chimeras also by performing microinjection of ES clones obtained by consortia such as KOMP and EUCOMM.

Types of mouse models:
  • Constitutive knock out
  • Conditional knock-out
  • Constitutive/Conditional Point mutation
  • Rosa26 knock-in
  • Humanization
  • Reporter fusions
  • Constitutive/Inducible Cre- or FLP-driver mouse strains

II. Zebrafish Service

During the last few decades, a unique set of properties has made the zebrafish a popular vertebrate model in various field of biology, ranging from developmental biology, human disease studies to environmental toxicology.
The Zebrafish Service provides expertise for transgenic lines and mutants generation and support for design and realization of toxicology studies with the zebrafish model.

Services available:
  • Zebrafish transgenesis by CRISPR/Cas9 technology
  • Morpholino oligonucleotide-mediated antisense gene-specific knockdowns
  • mRNA and cDNA-mediated gene misexpression
  • Expression pattern analysis of genes of interest (RNA in situ hybridization)
  • Important and rearing of mutant and transgenic lines
  • Embryonic phenotyping
  • Reproductive phenotyping
  • Animals treatment for toxicology studies under long term conditions